The Disk Membrane Bilayer Enhances Bovine Rhodopsin Kinetic Stability
نویسندگان
چکیده
منابع مشابه
Modulation of rhodopsin function by properties of the membrane bilayer.
A prevalent model for the function of rhodopsin centers on the metarhodopsin I (MI) to metarhodopsin II (MII) conformational transition as the triggering event for the visual process. Flash photolysis techniques enable one to determine the [MII]/[MI] ratio for rhodopsin in various recombinant membranes, and thus investigate the roles of the phospholipid head groups and the lipid acyl chains sys...
متن کاملRhodopsin in bilayer membranes.
Visual excitation is initiated with the absorption of light by the membrane-bound visual pigment rhodopsin. The effect of light on rhodopsin is now quite generally believed to be a photoisomerization of the chromophore, retinaldehyde, from the 1 1-cis to the all-rransconliguration(Hubbard & Kropf, 1957; Wald, 1968). This is followed by changes in the shape of the protein, and these lead to cond...
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Purified bovine rhodopsin was reconstituted into vesicles consisting of 1-stearoyl-2-oleoyl phosphatidylcholine or 1-stearoyl-2-docosahexaenoyl phosphatidylcholine with and without 30 mol % cholesterol. Rhodopsin stability was examined using differential scanning calorimetry (DSC). The thermal unfolding transition temperature (T(m)) of rhodopsin was scan rate-dependent, demonstrating the presen...
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Photoaffinity labeled (3diazoacetoxy)-9-CC-retinal (1) and (9-methylenediazoacetoxy)9-c&retinal (2) were synthesized and bound to bovine opsin to obtain visual pigment analogs having absorption maxima at 465 and 460 nm respectively. Binding studies established that synthetic retinals 1 and 2 bind to the natural binding site and that the integrity of the diazoacetoxy photoaffinity label is prese...
متن کاملIncorporation of membrane proteins into large single bilayer vesicles. Application to rhodopsin
A general procedure to incorporate membrane proteins in a native state into large single bilayer vesicles is described. The results obtained with rhodopsin from vertebrate and invertebrate retinas are presented. The technique involves: (a) the direct transfer of rhodopsin-lipid complexes from native membranes into ether or pentane, and (b) the sonication of the complex in apolar solvent with aq...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.1356